GENETIC ENGINEERING OF POINSETTIA WITH THE AIM OF ENHANCING ITS RESISTANCE TO POINSETTIA MOSAIC VIRUS
Poinsettia (Euphorbia pulcherrima) is a major ornamental pot plant in many countries. Two viruses, poinsettia mosaic virus (PnMV) and poinsettia cryptic virus, often infect modern poinsettia cultivars. To produce virus-resistant poinsettia plants, genetic engineering has been considered. Since there is no established method available for the transformation of poinsettia, we have chosen an electrophoresis-based transformation method to generate poinsettia transformants. The main advantage of this method is that we can avoid the time-consuming tissue culture and regeneration procedure. To develop a reliable transformation method, the green fluorescent protein (GFP) gene has been used as a reporter gene. Here we report our preliminary results.
Clarke, J.L., Klemsdal, S.S., Floistad, E., Hvoslef-Eide, A.K., Haugslien, S., Moe, R. and Blystad, D.R. (2006). GENETIC ENGINEERING OF POINSETTIA WITH THE AIM OF ENHANCING ITS RESISTANCE TO POINSETTIA MOSAIC VIRUS. Acta Hortic. 722, 321-325