ANDROGENESIS INDUCTION FROM TOMATO ANTHER CULTURES: CALLUS CHARACTERIZATION
Obtaining dihaploid homozygous lines through androgenesis induction is a promising alternative to the classical inbreeding and selection programs. However, this technology is still poorly developed in tomato. During androgenesis induction from cultured tomato anthers, a callus intermediate phase precedes organogenesis. Based on their origin, morphology, color, texture, internal architecture and ploidy levels, we classified the different calli observed into three different types. Type 1 originate from the connective tissue of the anther. Type 2 burst from the point of insertion of the anther filament. They may have a gametophytic origin, but never regenerate shoot apical meristems. Type 3 calli emerge from the line of dehiscence of the anther. Besides their putative haploid origin, type 3 calli are capable of regenerate shoot apical and root meristems. We propose a step of type 3 callus selection and enrichment to be included in order to optimize the protocol of dihaploid tomato plant regeneration from anther cultures.
Segui-Simarro, J.M. and Nuez, F. (2006). ANDROGENESIS INDUCTION FROM TOMATO ANTHER CULTURES: CALLUS CHARACTERIZATION. Acta Hortic. 725, 855-862
Lycopersicon esculentum, androgenesis, dihaploids, in vitro culture