FLAX PLANTS REGENERATED FROM UNPOLLINATED OVULES CULTURED IN OVARY SEGMENTS

Z. Bartosova, S. Masar, A. Pretova
We aimed to observe and to compare the induction rate of calli and regeneration rate of shoots originated from unpollinated ovules cultured in flax ovary segments. The percentage of induced calli in ovary culture varied from 15,4 in genotype AC Emerson to 32,4 in genotype Viking. Conversely the regeneration rate was higher in AC Emerson (8,8) rather than in genotype Viking (1,8). After four month in culture we screened for somaclonal variation in the calli. Flow cytometry analysis showed that the yellow-green calli in culture were mostly diploid in genotype Viking (83,3) and in AC Emerson (100). The dark green calli were of higher ploidy level and contained cells of 4n, 6n, 8n, 16n. Regeneration of shoots was performed exclusively from the diploid cells in the calli as we found out using karyological analysis on squashes on root tip cells. The origin of regenerated plants was proved using isozyme spectra analysis.
Bartosova, Z., Masar, S. and Pretova, A. (2006). FLAX PLANTS REGENERATED FROM UNPOLLINATED OVULES CULTURED IN OVARY SEGMENTS. Acta Hortic. 725, 869-879
DOI: 10.17660/ActaHortic.2006.725.120
https://doi.org/10.17660/ActaHortic.2006.725.120
somaclonal variation, gynogenesis, karyological analysis, flow-cytometry, isozyme
English

Acta Horticulturae