IN VITRO PLANT REGENERATION IN ZYGOTIC EMBRYO CULTURE OF IRIS REICHENBACHII
Plant regeneration from mature zygotic embryo culture of Iris reichenbachii was studied. The effect of 2,4-dichlorophenoxyacetic acid (2,4-D, 0-10 mg L-1) on induction of morphogenesis in vitro is investigated. The best response for callus induction is observed on MS medium with 10 mg L-1 2,4-D. White embryogenic, yellow non-embryogenic and green organogenic calli are observed. The fresh weight, as well as, percentage portion of embryogenic and organogenic calli is 2,4-D dependent. Shoots are succesfuly multiplied by adventitive organogenesis from organogenic callus. Germination of somatic embryos and rooting of shoots were achieved on MS solid medium without hormones.
Jevremonic, S., Subotic, A. and Radojevic, LJ. (2006). IN VITRO PLANT REGENERATION IN ZYGOTIC EMBRYO CULTURE OF IRIS REICHENBACHII. Acta Hortic. 725, 169-172
in vitro culture, iris