HISTOLOGY OF ADVENTITIOUS SHOOT PRODUCTION IN MICROPROPAGATED WALKING STICK CABBAGE (BRASSICA OLERACEA VAR. LONGATA)
Walking stick cabbage (Brassica oleracea var. longata) grows tall, vertical stems up to two metres high and is used as an ornamental plant as well as producing foliage that can be used as a fodder crop. Further, the dried stems provide raw materials for the production of crafted walking sticks. A protocol for in vitro propagation of walking stick cabbage was established using nodal sections of hypocotyl from 2 cm, newly-germinated seedlings. Adventitious shoots developed from stem callus after 2-week growth on MS medium supplemented with BA at 16 mg/L, maximum shoot regeneration being achieved with 4 mg/L of BA. As many as 25 new shoots can be produced from a callusing explant at each culture passage. Histological studies showed that promeristems were initiated from a single callus cell, subsequently developing into active meristems at the callus surface. These extended to produce clustered adventitious shoots that could be transferred to fresh medium for acclimation.
Zhao, Y., Green, J., Yu, M. and Grout, B.W.W (2006). HISTOLOGY OF ADVENTITIOUS SHOOT PRODUCTION IN MICROPROPAGATED WALKING STICK CABBAGE (BRASSICA OLERACEA VAR. LONGATA). Acta Hortic. 725, 231-234
Brassica longata, micropropagation, adventitious shoots