THE USE OF MICROARRAY µPEACH 1.0 TO INVESTIGATE THE TRANSITION FROM PRE-CLIMACTERIC TO CLIMACTERIC PHASE IN PEACH FRUIT
The transition from preclimacteric to climacteric phase is a critical step during fruit development and ripening. An holistic approach to study this transition has been undertaken using the first peach microarray (µpeach 1.0) containing 4800 oligos corresponding to an equal number of genes expressed at preclimacteric and climacteric stages. Microarray hybridization indicated that 260 and 98 genes are up- and down-regulated, respectively. According to the TAIR Gene Ontology, genes have been classified into three main ontologies named cellular localization, molecular function and biological process. Considering the cellular localization the most significant up-and down-regulated gene products belong to cell wall and chloroplast. As far as cell wall is concerned major up-regulated genes encode pectinesterases and expansins, while down-regulation regards genes involved in cell wall growth and lignification. Among genes localized at chloroplast level a dramatic up-regulation of those controlling isoprenoids biosynthesis has been observed. Considering ontologies concerning molecular functions and biological process, a dramatic up-regulation has been detected for genes encoding transcription factors and enzymes involved in ethylene biosynthesis and action. Among transcription factors members of the APETALA and AUX/IAA families are the most represented, while within the family of ethylene receptors a peach new member has been described.
Bonghi, C. and Ramina, A. (2006). THE USE OF MICROARRAY µPEACH 1.0 TO INVESTIGATE THE TRANSITION FROM PRE-CLIMACTERIC TO CLIMACTERIC PHASE IN PEACH FRUIT. Acta Hortic. 727, 25-36
Ethylene, gene expression profiling, isoprenoids, Prunus persica, ripening, softening, transcription factors