FIRST LINKAGE MAPS OF ARTICHOKE USING PCR-DERIVED MARKERS
We report on the construction of the first linkage maps in Cynara cardunculus var. scolymus (2n=2x=34)by applying a combination of PCR-based markers. A segregating F1 population of 94 individuals was produced by crossing a late-maturing, non-spiny type (Romanesco C3) and an early-maturing spiny type (Spinoso di Palermo). Linkage analysis was carried out by applying the double pseudo-testcross strategy. Two genetic maps were constructed: one for each parent. A total of 443 markers (352 AFLP, 12 SSR, 38 M-AFLP and 41 S-SAP) were used in linkage analysis. The female parent map consists of 204 markers mapped on 18 major linkage groups, spanning a total length of 1330.5 cM, with an average distance of 6.5 cM between adjacent markers. The male parent map has 181 markers ordered in 17 major groups, covering 1239.7 cM, with a marker density of 6.9 cM. In addiction 7 and 6 minor groups (triplets and duplets) were obtained for the female and male map respectively. The two maps were joined by using 72 common markers, which allowed the identification of 16 homologous linkage groups. The number of major linkage groups correspond, for the male map, to the artichoke haploid chromosomal number (17). The maps generated provide the basis for future mapping of agriculturally relevant traits.
Portis, E., Acquadro, A., Magurno, F., Lanteri, S., Mauromicale, G. and Mauro, R. (2007). FIRST LINKAGE MAPS OF ARTICHOKE USING PCR-DERIVED MARKERS . Acta Hortic. 730, 67-73
molecular markers, linkage map, AFLP, M-AFLP, S-SAP, microsatellite