DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS
By using the coat protein gene of WMV-2, and replicase genes of ZYMV and CMV, a trivalent expression vector was constructed for transformation of watermelon plants with Agrobacterium tumefaciens. The transgenes were confirmed in progenies of T1, T2 and T3 generations by PCR-Southern blot analysis. By mechanical inoculation in greenhouse and field trial, the transgenic lines showed resistance to virus infections in relatively high level and the resistant transgenic line BH-1 was selected successfully.
Sheng-Niao, N., Xue-Sen, H., Sek-Man, W., Jia-Lin, Y., Fu-xin, Z., Sen-you, W., Guang-Ming, Z., Fan-Sheng, S., Xiao-Yu, C., Yong-Gang, C., Yan, X., Fang, Z. and Xiao-Hong, W. (2007). DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS. Acta Hortic. 731, 71-76
watermelon, virus diseases, gene transformation, resistance to virus infection