DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS

N. Sheng-Niao, H. Xue-Sen, W. Sek-Man, Y. Jia-Lin, Z. Fu-xin, W. Sen-you, Z. Guang-Ming, S. Fan-Sheng, C. Xiao-Yu, C. Yong-Gang, X. Yan, Z. Fang, W. Xiao-Hong
By using the coat protein gene of WMV-2, and replicase genes of ZYMV and CMV, a trivalent expression vector was constructed for transformation of watermelon plants with Agrobacterium tumefaciens. The transgenes were confirmed in progenies of T1, T2 and T3 generations by PCR-Southern blot analysis. By mechanical inoculation in greenhouse and field trial, the transgenic lines showed resistance to virus infections in relatively high level and the resistant transgenic line BH-1 was selected successfully.
Sheng-Niao, N., Xue-Sen, H., Sek-Man, W., Jia-Lin, Y., Fu-xin, Z., Sen-you, W., Guang-Ming, Z., Fan-Sheng, S., Xiao-Yu, C., Yong-Gang, C., Yan, X., Fang, Z. and Xiao-Hong, W. (2007). DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS. Acta Hortic. 731, 71-76
DOI: 10.17660/ActaHortic.2007.731.8
https://doi.org/10.17660/ActaHortic.2007.731.8
watermelon, virus diseases, gene transformation, resistance to virus infection
English
https://doi.org/10.17660/ActaHortic.2007.731.8
731_8
71-76
 

Acta Horticulturae