THE DEVELOPMENT OF A POLYMERASE CHAIN REACTION METHOD FOR THE RAPID IDENTIFICATION OF GRAPE PHYLLOXERA IN VINEYARD SOIL
Grapevine phylloxera, Daktulosphaira vitifoliae poses a serious threat to around 85% of vineyards in Australia. Phylloxera interaction with the root system of ungrafted European grapevines results in severe yield reduction and eventual vine death. Crucial to the effectiveness of Australian quarantine protocols is the early detection and containment of new outbreaks of phylloxera. A combination of aerial and ground surveys are currently in place to detect new infestations and monitor the spread of phylloxera infestations in Australia. Several limitations exist with these early detection methods making an accurate and thorough assessment of phylloxera damage in a vineyard difficult. There is an urgent need to develop new detection systems including those able to directly recognise the insect itself and not its associated symptoms on grapevines. This paper reviews current early detection approaches with an emphasis on detection of phylloxera using a polymerase chain reaction (PCR)-based technology. The aim of this research is to develop a species-specific molecular diagnostic for the rapid detection of phylloxera in vineyard soils.
Herbert, K.S., Hoffmann, A.A., Powell, K.S., Ophel-Keller, K. and Parsons, Y.M. (2007). THE DEVELOPMENT OF A POLYMERASE CHAIN REACTION METHOD FOR THE RAPID IDENTIFICATION OF GRAPE PHYLLOXERA IN VINEYARD SOIL. Acta Hortic. 733, 75-88
grape phylloxera, Daktulosphaira vitifoliae, polymerase chain reaction, molecular diagnostics, insects, DNA barcode