INHIBITORY EFFECT OF SAFFRON (CROCUS SATIVUS L.) FROM DIFFERENT COUNTRIES
Saffron (Crocus sativus L.) from ancient times has been used as a medicinal herb. Today, as a rich source of carotenoids, saffron is known for its anti-cancer and anti-tumor effects. It was known that estimated chemical composition and concentrations of main biological active ingredients of saffron strongly depended on the method employed for cultivation, drying, extraction, separation, quantification and climatic conditions of origin place. The present comparative study was designed to evaluate the cytotoxic activity of saffron from 11 different sources (Azerbaijan; China; France; Greece; India; Iran; Italy; New Zealand; Spain; Turkey and Sigma company) against HeLa human malignant cells (epitheloid carcinoma-cervix). The cytotoxic effects of these extracts were determined by using viability and colony formation assays. All used saffron extracts had no cytotoxic effect on normal human cells. The IC50 of saffron extracts on viability of HeLa cells varied from 2.5 to 5.3 mg/ml. The IC50 of saffron extracts on colony formation of HeLa cells varied between different sources from 0.353 to 0.740 mg/ml. In both assays, saffron from India possesses more inhibitory activity and saffron from China less inhibitory activity. Possible explanation for differential cytotoxic activity of used saffron extracts against human malignant cells may lay in the difference in chemical composition and concentrations of biological active carotenoid ingredients of saffron from different sources. Currently, this difference in cytotoxic activity of saffron from different sources is under investigation.
Riverón-Negrete, L., Córdova, J.P. and Abdullaev, F. (2007). INHIBITORY EFFECT OF SAFFRON (CROCUS SATIVUS L.) FROM DIFFERENT COUNTRIES. Acta Hortic. 739, 297-302
viability assays, cytotocity, cancer cells, biological active, anti-cancer