PRODUCTION OF MARKER-FREE INSECT RESISTANT TRANSGENIC BRINJAL (SOLANUM MELONGENA L.) CARRYING CRY2AB GENE

M. Narendran, Seema Lalwani, Prakash Girhepuje, Dattatray Shirale, Ghulam Mustafa, Ratnapal Gandhi, Srinivas Parimi, Bharat R. Char, Usha B. Zehr
We used Agrobacterium-mediated co-transformation for the generation of marker-free insect resistant transgenic plants for fruit and shoot borer (Leucinodes orbonalis) expressing the cry2Ab gene. One T-DNA contained the cry2Ab gene borne on one plasmid and the other T-DNA contained nptII and GUS marker genes borne on another plasmid. These two plasmids were both introduced into the Agrobacterium strain LBA4404. A large number of transgenic plants were produced carrying both T-DNAs. These plants were screened by double antibody sandwich ELISA for the detection of Cry2Ab protein and GUS assays for expression of β-glucuronidase. The cry2Ab and marker genes were found to have segregated independently in 44% of the primary transformants analyzed, indicate that the T-DNA insertions were unlinked. The marker-free status of the plants was confirmed by molecular analysis and germination tests in vitro on kanamycin-containing medium. Transformants that displayed normal Mendelian inheritance were chosen for further analysis. The shoots and fruits of a number of marker-free transgenic plants were screened using larvae of fruit and shoot borer (Leucinodes orbonalis).
Narendran, M., Seema Lalwani, , Prakash Girhepuje, , Dattatray Shirale, , Ghulam Mustafa, , Ratnapal Gandhi, , Srinivas Parimi, , Bharat R. Char, and Usha B. Zehr, (2007). PRODUCTION OF MARKER-FREE INSECT RESISTANT TRANSGENIC BRINJAL (SOLANUM MELONGENA L.) CARRYING CRY2AB GENE. Acta Hortic. 752, 535-538
DOI: 10.17660/ActaHortic.2007.752.100
https://doi.org/10.17660/ActaHortic.2007.752.100
Agrobacterium, Bacillus thuringiensis, co-transformation, eggplant,
English

Acta Horticulturae