DNA FINGERPRINTING OF KIWIFRUIT (ACTINIDIA SPP.) GENOTYPES USING FLUORESCENT AMPLIFIED FRAGMENT LENGTH POLYMORPHISM

The value of fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting for identifying genotypes and analyzing genetic variation in genus Actinidia was assessed. Characterization of FAFLP markers and their use in fingerprinting 31 kiwifruit genotypes grown in Jiangxi Province, China is reported. Of the eight primer pairs assessed, four (E-AAC + M-CAC, E-AAG + M-CTG, E-AAC + M-CAG and E-AAC + M-CTA) were screened because of the number and quality of polymorphic fragments they produced. A total of 190 FAFLP markers were scored using the four primer pairs. 179 (94.2%) were polymorphic and showed a high level of genetic diversity. All kiwifruit genotypes tested could be uniquely identified using the four primer pairs.