R. Lengliz, H. Majoul, S. Gharsallah-Chouchane, F. Gorsane, H. Fakhfakh, M. Marrakchi
This paper reports on protocols for in vitro plant regeneration and gene transfer for PVMV resistance from two accessions of Lycopersicon esculentum Mill. Shoot organogenesis was achieved on media containing Zeatin/IAA combination. The two accessions tested, Riogrande and Justar, showed a distinct differences in both their aptitude to regeneration and transformation, a high success rate was obtained for the first genotype (Riogrande) in every stage of the process. When shoot induction media was supplemented with timentin (500 mgL-1), a high regeneration frequency and a great number of shoots were obtained. It was observed that timentin induced an increase in the morphogenesis of in vitro tomato explants. Our results confirm those of a previous investigation on the beneficial effects of this class of antibiotics on tomato regeneration and consequently, its reliability for use in the transformation of this species. Kanamycin resistant plants were obtained and the first selfed progenie of Riogrande and Justar transgenic plants was characterized and their level of resistance to PVMV was evaluated in the greenhouse.
Lengliz, R., Majoul, H., Gharsallah-Chouchane, S., Gorsane, F., Fakhfakh, H. and Marrakchi, M. (2007). FACTORS AFFECTING TOMATO (LYCOPERSICON ESCULENTUM MILL.) TRANSFORMATION FREQUENCY . Acta Hortic. 758, 43-51
DOI: 10.17660/ActaHortic.2007.758.3
Lycopersicon esculentum, regeneration, transformation, timentin, zeatin

Acta Horticulturae