APPLICATION OF SUPPRESSIVE SUBTRACTION HYBRIDIZATION IN THE STUDY OF EMBRYO ABORTION RELATED TO GENE EXPRESSION IN LITCHI (LITCHI CHINENSIS SONN.)

Xiang Xu, Zhang YiShun, Huang ShangZhi, Ou Liangxi, Qiu Yanping, Li Zhiqiang, Chen Jiezhen
To study the molecular mechanisms associated with embryo abortion in seed-shriveled litchi varieties, suppressive subtraction hybridization (SSH) was used to investigate differential gene expression of normal-seeded and seed-shriveled embryos during stages of I to II of fruit-set. The differential expression cDNA library, representing the specific cDNA for aborting embryos of 'GuiWei' litchi, was built using the embryos from different fruit-set stages, with the normal-seeded embryo as the driver and the seed-shriveled embryo as the tester. After testing by Virtual Northern, 3 positive clones were obtained. We isolated 3 cDNAs from the 3 clones, which were 342, 599 and 391 nucleotides long and were designated Lce1, Lce2 and Lce3, respectively. The Lce1 amino acid sequence showed 97% homology with ubiquitin-conjugated protein of Capsicum and Solanum, Lce2 showed 94% homology with a Brassica S-adenosylmethionine synthetase and Lce3 showed 98% homology with a Zea mays methionine adenosyltransferase. These are the first reported sequences in litchi. The full-length sequence was obtained by PCR amplification and a complete cDNA for SAM synthetase gene was obtained. The sequence was 1515 base pairs (bp) with an opening reading frame (ORF) encoding 393 amino acids (GenBank accession NO: AY259227). This SAM synthetase gene in embryos of 'Guiwei' litchi may be associated with abortion.
Xiang Xu, , Zhang YiShun, , Huang ShangZhi, , Ou Liangxi, , Qiu Yanping, , Li Zhiqiang, and Chen Jiezhen, (2007). APPLICATION OF SUPPRESSIVE SUBTRACTION HYBRIDIZATION IN THE STUDY OF EMBRYO ABORTION RELATED TO GENE EXPRESSION IN LITCHI (LITCHI CHINENSIS SONN.). Acta Hortic. 763, 83-90
DOI: 10.17660/ActaHortic.2007.763.11
https://doi.org/10.17660/ActaHortic.2007.763.11
virtual northern blot, S-adenosylmethionine synthetase gene (SAM gene)
English

Acta Horticulturae