DEVELOPMENT OF MOLECULAR MARKERS LINKED TO THE TRAITS OF NON-ACID/ ACID FRUIT OF PEACH

Jun Wu, Huairui Shu, Kaichun Zhang, Lijie Jiang
AFLP and bulked segregant analysis were used to screen markers linked to the non-acid/acid trait of peach fruit controlled by D/d gene. Of 64 pairs of primer combinations, twelve amplified polymorphic DNA fragments between non-acid bulk and acid bulk. Only two specific fragments were linked to acid trait (recessive d location), after investigating the progenies. Specific fragments TA-CTC140 and AT-CTA200 were derived from primers E-TA/M-CTC and E-AT/M-CTA. The linkage distances to D/d gene were 1.47cM and 2.99cM, respectively. Specific primers BFPA1/A2 and BFPB1/B2 were designed according to the sequences of two specific fragments. With primers BFPB1/B2, genomic DNA amplified the polymorphic band AT-CTA158 in acid bulks and individuals. The AFLP marker AT-CTA200 was converted into a SCAR marker. The linkage distance of marker AT-CTA158 to d gene was the same as that of the original AFLP marker. However, the AFLP marker TA-CTC140 was not converted into a SCAR marker. Polymorphism disappeared in both parents and progenies when amplified with primer pair A1/A2.
Jun Wu, , Huairui Shu, , Kaichun Zhang, and Lijie Jiang, (2007). DEVELOPMENT OF MOLECULAR MARKERS LINKED TO THE TRAITS OF NON-ACID/ ACID FRUIT OF PEACH. Acta Hortic. 763, 131-138
DOI: 10.17660/ActaHortic.2007.763.17
https://doi.org/10.17660/ActaHortic.2007.763.17
Prunus persica (L.) Batsch, AFLP, SCAR
English

Acta Horticulturae