RESPONSE TO SUCROSE-FREE CULTURE AND DIFFUSIVE VENTILATION OF PLANTLETS IN VITRO OF GERBERA JAMESONII AND PHOTOAUTOTROPHIC GROWTH POTENTIAL

FeiXiong Liao, BiQing Wang, MengJin Zhang, FangNi Xu, FangQin Lian
Regenerated shoots in vitro of Gerbera jamesonii Boul. were classified into three categories according to the height and leaf number and transferred into 250 ml glass vessels with 1/2MS supplemented with 6 g Agar/L, and 30 g sucrose/L or sucrose-free as rooting medium. The vessels were capped with Polypropylene film as the closed or with polypropylene film embedding a round gas-permeable membrane as diffusive ventilation. Three categories of the shoots could survive in the sucrose-free medium and grew photoautotrophically. The fresh weight and dry mass percentage of the shoots under diffusive ventilation were significantly higher than those under the closed. The fresh weight and dry mass percentage of those shoots 2–3 cm height with 3–4 leaves were 4.32 g and 10.6%, which were the highest value among the three categories. The root number and length of the plantlets in category II were about 4 and 3.0 cm on average respectively also the highest value among the three categories. Diffusive ventilation of the vessel could increase the chlorophyll content which of the plantlets in category II were 148.2 and 63.5 µg.g-1 FW respectively for chlorophyll a and b, which was higher significantly than those in other categories. The maximal quantum yield (Фpmax ) and the actual quantum yield (Фp) for the plantlets cultured under diffusive ventilation were significantly higher than those for plantlets under the closed. Фpmax cultured under diffusive ventilation was 0.8 and Фp for the plantlets in category II was 4.32 significantly higher than those in other categories.
FeiXiong Liao, , BiQing Wang, , MengJin Zhang, , FangNi Xu, and FangQin Lian, (2007). RESPONSE TO SUCROSE-FREE CULTURE AND DIFFUSIVE VENTILATION OF PLANTLETS IN VITRO OF GERBERA JAMESONII AND PHOTOAUTOTROPHIC GROWTH POTENTIAL. Acta Hortic. 764, 257-264
DOI: 10.17660/ActaHortic.2007.764.34
https://doi.org/10.17660/ActaHortic.2007.764.34
micropropagation, photoautotrophic growth, environmental control, cut flower, chlorophyll content, chlorophyll photochemistry
English

Acta Horticulturae