SOMATIC EMBRYOGENESIS IN CIMICIFUGA RACEMOSA
Since a long time Cimicifuga has been used for therapeutic purposes. The demand for the plant drug Cimicifugae rhizome has been increased tremendously during the last years. This study aimed to develop a method for somatic embryogenesis in Cimicifuga. Hypocotyl segments were used as explants and cultivated for 2 to 18 weeks on a modified medium according to Murashige and Skoog containing 1 mg L-1 2,4 D. Embryogenic callus occurred after 2 weeks. After transferring this callus onto a hormone-free MS medium with 2.5 g L-1 activated charcoal, somatic embryos were formed and matured. Mature embryos were separated and continued growing on the hormone-free medium. After two months from 100 embryos 80 plantlets were formed but only about 44 of these plantlets could be transplanted after 6 months. The plantlets were cultivated till a size of 3 cm in vitro and than transplanted in a peat substrate for acclimatization. The acclimatized plantlets were transferred into the greenhouse. Plants of a size of 20 cm were planted in the open field. Since 3 years plants derived from somatic embryos have been cultivated in the field and evaluated concerning morphological and physiological properties.
Pinker, I. and Schenk, R. (2007). SOMATIC EMBRYOGENESIS IN CIMICIFUGA RACEMOSA. Acta Hortic. 764, 265-270
Ranunculaceae, in vitro propagation, histology