ADVANCED CELL CULTURE TECHNOLOGY FOR ESSENTIAL OIL PRODUCTION AND MICROARRAY STUDIES LEADING TO DISCOVERY OF GENES FOR FRAGRANCE COMPOUNDS IN MICHELIA ALBA (CEMPAKA PUTIH)

R. Ibrahim, S. Hussein, N. Noordin, E. Azlan, M.A. Manan, H. Adnan, M. Vellupillai
Studies were conducted to develop an efficient cell culture system for the production of callus, suspension cell cultures, and somatic embryos from Michelia alba and for the production of essential oils using advanced bioreactor technology. Direct somatic embryogenesis had been successfully obtained from petal explants using solid MS media containing 2,4-D. On the other hand, MS media containing BA produced both callus and globular structures of somatic embryos within 10–15 days of incubation. One major problem anticipated in this approach, based on our early experiments, is limited or minimal amount of fragrance produced in the cultures. The appropriate strategy is to superimpose DNA microarray studies on a cell culture project. The study covers natural flower development phases and led to the identification of genes or sets of genes that regulate the production of the fragrance. Seven developmental stages of Michelia alba (‘Cempaka putih’) flowers, namely Stage 5 (S5) to 11 (S11), were investigated for their volatile constituents. The essential oil was isolated by a Simultaneous Distillation Extraction (SDE) technique, and the oil obtained was subjected to GC-MS analysis. In total, seventy-seven compounds (77), representing 93–98% of the overall Michelia alba volatiles, were identified on the basis of mass spectra and retention indices. Thirty-three (33) of these compounds belong to the isoprenoid group, which comprised 30–50% of the total volatile compounds; whereas, the remaining were fatty acid derivatives and benzenoid, phenylpropanoid, and other hydrocarbon compounds.
Ibrahim, R., Hussein, S., Noordin, N., Azlan, E., Manan, M.A., Adnan, H. and Vellupillai, M. (2008). ADVANCED CELL CULTURE TECHNOLOGY FOR ESSENTIAL OIL PRODUCTION AND MICROARRAY STUDIES LEADING TO DISCOVERY OF GENES FOR FRAGRANCE COMPOUNDS IN MICHELIA ALBA (CEMPAKA PUTIH). Acta Hortic. 765, 95-100
DOI: 10.17660/ActaHortic.2008.765.11
https://doi.org/10.17660/ActaHortic.2008.765.11
bioreactor, floral scent, gene expression, somatic embryogenesis, volatile compounds
English

Acta Horticulturae