GENETIC PURITY TEST OF F1 HYBRID TOMATO USING MOLECULAR MARKER ANALYSIS

Three DNA molecular marker systems, RAPD, ISSR and SSR, were used to test seed genetic purity of a hybrid tomato ‘Hezuo906’. Genetic relationships of the F₁ hybrid and their two parents were analyzed with 218 RAPD random decamer primers, 54 ISSR primers, and 49 SSR primers. Among the 321 primers screened, two dominant RAPD primers and two codominant SSR primers were selected for testing the genetic purity of the F₁ seeds. A total of 208 hybrid individuals were investigated with the selected primers. The combined results of the marker analyses confirmed that ten of 208 ‘Hezuo906’ were false hybrids, and the overall genetic purity of the F₁ hybrid seed lot was 95.1%. This study showed that the molecular markers are highly efficient and reproducible for genetic purity testing of tomato commercial hybrid seeds.