SELECTION OF METARHIZIUM ANISOPLIAE AND BEAUVERIA BASSIANA ISOLATES TO CONTROL ASYNONYCHUS CERVINUS AND OTIORHYNCHUS SULCATUS (COLEOPTERA: CURCULIONIDAE) ATTACKING RASPBERRIES IN CHILE

M. Gerding, M. Rodríguez, A. France, M. Gerding-González
Two of the main insect pests of raspberries in Chile are the two curculionids: Asynonychus cervinus (Fuller rose beetle) and Otiorhynchus sulcatus (Black vine weevil). At the larval stage they feed on the roots of the crop causing an important economic damage. We carried out a screening of Beauveria bassiana and Metarhizium anisopliae isolates to determine their pathogenicity on A. cervinus and O. sulcatus, both at adult and larval stage. Thirty seven B. bassiana isolates were evaluated on A. cervinus larvae and 24 M. anisopliae isolates on O. sulcatus adult and larvae. The selected isolates were tested by dipping individuals of each specie in suspensions of different conidia concentrations (0 to 108 conidia ml-1). Mortality rates, Lethal Concentrations (LC50 and LC90) and food intake were determined. Different levels of pathogenicity among the isolates were detected. The most aggressive B. bassiana isolates for A. cervinus were Qu-B179, Qu-B299, Qu-B314 and Qu-B323. For O. sulcatus adults, the most virulent M. anisopliae isolates were Qu-M151b, Qu-M232 and Qu-M271. The best isolate to control O. sulcatus was Q-M151b, with a LC50 of 1x106.53 and LC90 of 1x107.82 conidia ml-1. Adult A. cervinus leaf consumption after B. bassiana inoculation was reduced up to 70% in 35 days. The LC50 and LC90 calculated for A. cervinus were 1x104.7 and 1x108.3 conidia ml-1, respectively.
Gerding, M., Rodríguez, M., France, A. and Gerding-González, M. (2008). SELECTION OF METARHIZIUM ANISOPLIAE AND BEAUVERIA BASSIANA ISOLATES TO CONTROL ASYNONYCHUS CERVINUS AND OTIORHYNCHUS SULCATUS (COLEOPTERA: CURCULIONIDAE) ATTACKING RASPBERRIES IN CHILE . Acta Hortic. 777, 391-396
DOI: 10.17660/ActaHortic.2008.777.59
https://doi.org/10.17660/ActaHortic.2008.777.59
biological control, curculionids, entomopathogenic fungus, biopesticide
English

Acta Horticulturae