EFFECT OF COPPER AND GLUCOSE ON ROOT DEVELOPMENT AND CORM ANTI-OXIDATIVE STRESS ENZYMES ACTIVITY IN SAFFRON (CROCUS SATIVUS L.)
Root elongation, root number and corm enzymatic activity were studied in Crocus sativus L. cultivated in the presence of increasing copper or glucose concentrations. Root elongation was moderately stimulated in up to 0.00002% copper, but reduced in copper concentrations ranging from 0.0001% to 1%. After 6 days cultivation, root length was only 25% of the control in 0.001% copper and was negligible in 1% copper. The number of roots per corm remained unchanged in up to 0.5% copper, but rooting was aborted in 1% copper. Catalase activity doubled in 0.005% copper, decreased by 30% in 0.2% copper, and became undetectable in higher copper concentrations. Peroxidase activity increased by 30% in 0.00001 to 0.01% copper, but was halved in 1% copper. Superoxide dismutase activity rose to 300% in 0.02% copper, but was reduced to 75% in 1% copper. After 24 hours cultivation in the presence of glucose, root length was the same in the control and in up to 1% glucose, but was only 30% of the control in 20% glucose. After 6 days, root length was 35% of the control in 0.5% glucose and 5% of the control in 20% glucose. Root number was roughly the same in up to 5% glucose, and reached 80% of the control after 6 days in 10 and 20% glucose. Catalase activity rose to 200% in corms cultivated in 1% glucose and was still 125% in corms cultivated in 20% glucose. Peroxidase activity was up to 160% in corms cultivated in 20% glucose. Superoxide dismutase rose to 220% and 750% of the control value in corms cultivated in 10 and 20% glucose, respectively. Thus while rooting was particularly sensitive to changes in the growing medium, the saffron corm attempted to adjust its enzymatic activity in response to environmental stresses.
Keyhani, E. and Keyhani, J. (2008). EFFECT OF COPPER AND GLUCOSE ON ROOT DEVELOPMENT AND CORM ANTI-OXIDATIVE STRESS ENZYMES ACTIVITY IN SAFFRON (CROCUS SATIVUS L.). Acta Hortic. 779, 677-688
cultivation media, root number, root length, catalase, peroxidase, superoxide dismutase