I.E. Tzanetakis, A. Halgren, R.R. Martin
During routine graft indexing of Rubus clones at the USDA-ARS Horticultural Crops Research Laboratory in Corvallis, Oregon, a 'Glen Clova' plant that originated in Scotland induced severe symptoms including stunting and apical necrosis when grafted on 'Munger' black raspberry (Rubus occidentalis). Cloning of viral dsRNA obtained from the plant and sequence analysis indicated the presence of a novel virus, a member of the Closteroviridae and possibly additional viruses. Detection protocols developed against the closterovirus were used and identified the virus in breeding and field material from Washington State as well as several additional raspberry clones from Scotland. Graft indexing of selection 'WSU-991' on R. Occidentalis induced mottling symptoms and was free of other known raspberry viruses. The virus was thus temporarily designated as Raspberry mottle virus (RMoV). The complete nucleotide sequence of the virus has been obtained and exceeds 17 kilobases. Phylogenetic analysis indicated that RMoV is closely related to members of the Closterovirus genus, the aphid-borne closteroviruses. The genome organization is an intermediate between Citrus tristeza virus and Beet yellows virus with two putative leader proteases and a conserved array of open reading frames at the 3' proximal terminus. Currently, additional graft indexing tests are in progress to examine the possibility that RMoV is the causal agent of Raspberry leaf mottle or Raspberry leaf spot diseases previously identified in Rubus.
Tzanetakis, I.E., Halgren, A. and Martin, R.R. (2008). COMPLETE NUCLEOTIDE SEQUENCE AND PROPERTIES OF RASPBERRY MOTTLE VIRUS. Acta Hortic. 780, 35-40
DOI: 10.17660/ActaHortic.2008.780.4
Rubus, Closterovirus, Closteroviridae, transmission, detection