FRUIT QUALITY OF TRANSGENIC 'MEEKER' RED RASPBERRY WITH RESISTANCE TO RASPBERRY BUSHY DWARF VIRUS

S.M.M. Malowicki, M.C. Qian, R.R. Martin
Raspberry bushy dwarf virus (RBDV) causes significant reduction in yield and crumbly fruit in raspberries, blackberries and raspberry-blackberry hybrids; the only means of control is through development of resistant cultivars. Genetic modifications have been made to 'Meeker' red raspberries in an effort to develop resistance to RBDV while maintaining the desirable fruit characteristics of 'Meeker' including sweetness, aroma profile and machine harvestability. Transgenic and wild-type 'Meeker' plants were grown in Oregon and Washington to compare the fruit quality under different climatic conditions over several years and to evaluate field resistance to RBDV. After six field seasons, only one of the transgenic lines was still completely free of RBDV under extreme disease pressure. The wild-type 'Meeker' plants in the same plots were 100% infected (202/202) after three field seasons. This transgenic line along with four other lines that were less than 100% infected after six field seasons was not infected with RBDV by grafting after three attempts. Fruit quality indicators such as °Brix, titratable acidity and sugar and organic acid profiles were performed on fruit from wild-type and five transgenic lines of 'Meeker' raspberries grown in Oregon and Washington for the years 2004 and 2005. Significant differences (p<0.05) were found between the years for all tests performed. °Brix and titratable acidity were also significantly affected by locations with berries grown in Washington having higher acid and lower °Brix than Oregon. The RBDV-resistant transgenic lines were similar to the wild-type 'Meeker' red raspberry. Experimental results showed that fruit quality is affected by season and growing environments. Additionally, the five transgenic lines behaved similarly to the wild-type 'Meeker' raspberry in yield. These results demonstrated that the transgenic RBDV-resistant lines of 'Meeker' could serve as a replacement for the wild-type 'Meeker' red raspberry provided that the fruit was accepted in the marketplace.
Malowicki, S.M.M., Qian, M.C. and Martin, R.R. (2008). FRUIT QUALITY OF TRANSGENIC 'MEEKER' RED RASPBERRY WITH RESISTANCE TO RASPBERRY BUSHY DWARF VIRUS. Acta Hortic. 780, 41-48
DOI: 10.17660/ActaHortic.2008.780.5
https://doi.org/10.17660/ActaHortic.2008.780.5
RBDV resistance, fruit quality, red raspberry, transgenic, volatile, SBSE
English