DETECTION AND SEQUENCE COMPARISON OF THE BLACKCURRANT REVERSION VIRUS IN BLACK, RED AND WHITE CURRANT
We collected samples from black, red and white currants (Ribes spp.) showing symptoms of blackcurrant reversion disease (severe form) and full blossom disease (FBD), cultivated in the Czech Republic. We detected 15 new isolates of the Blackcurrant reversion virus (BRV) with RT-PCR and two sets of primers (Lemmetty et al., 1998, 2001). After amplification, a substantial part of the 3´ untranslated region (UTR) of BRV RNA 2 was sequenced and analyzed. The nucleotide sequence identity among the Czech isolates ranged from 91.9 to 99.8%. Four branches, each consisting of 6 to 9 isolates, occurred on the phylogenetic tree created after the analysis of the new and all other available BRV sequences. No correlation with the geographic origin was visible on the tree - Czech and Polish isolates occurred in all four branches. We found no correlation between the host and the topology of the tree. Black currant isolates occurred in branches 3 and 4 (with one exception in each branch). Isolates from black, red and white currants occurred in all four branches. No correlation between the sequence, symptoms and their severity was recorded.
Přibylová, J., Špak, J., Petrzik, K., Kubelková, D. and Špaková, V. (2008). DETECTION AND SEQUENCE COMPARISON OF THE BLACKCURRANT REVERSION VIRUS IN BLACK, RED AND WHITE CURRANT. Acta Hortic. 780, 61-66
Blackcurrant reversion virus, currant, RT-PCR