SURVEY, PHENOLOGIC DEVELOPMENT AND MOLECULAR CHARACTHERIZATION OF CHESTNUT TRADITIONAL VARIETIES FROM TERCEIRA ISLAND MADE BY GERMOBANCO III PROJECT

L. Monteiro, D.J.H. Lopes, P. Monjardino, M.S. Lopes, C. Baptista, D. Mendonça, C. Carvalho, A. Domingues, M. Melo, A. da Câmara Machado
Given the great importance of chestnut production in some islands of the Azores Archipelago, and because since the XIX century this plant has been cultivated on the islands an investigation started with the major goal of studying the actual state of this culture, trying to identify potential chestnut regional varieties, using a phenologial, phenotypic and a molecular characterization of all the samples collected from the field. All this work was carried out with regional cultivars of Castanea sativa and began with the localization and identification of the potential chestnut regional cultivars by making direct inquiries to producers and using GPS to define their location. The collected data were analysed in an ArcView environment using digital terrain photos that built a GIS map with the exact location of all the samples made. From this collecting work it was possible to register in a project database 92 chestnut entries. Because we are dealing with a plant in which the producer normally uses a vegetative propagation method to get new chestnut plants, all the collected material was propagated in vivo in collection fields created by this project. Concerning the characterization of possible different chestnut cultivars a morphological characterization was made, following up some international phenotypic and phenological describers (IPGRI). Also in the present study, 46 chestnut accessions were genotyped with 5 microsatellite loci in order to discriminate them. That permitted us to identify 31 different genotypes. Of these samples, 42 were from Terceira Island and 4 from São Miguel Island. A total of 51 alleles were detected, with a mean of 10.2 alleles per locus.
Monteiro, L., Lopes, D.J.H., Monjardino, P., Lopes, M.S., Baptista, C., Mendonça, D., Carvalho, C., Domingues, A., Melo, M. and da Câmara Machado, A. (2008). SURVEY, PHENOLOGIC DEVELOPMENT AND MOLECULAR CHARACTHERIZATION OF CHESTNUT TRADITIONAL VARIETIES FROM TERCEIRA ISLAND MADE BY GERMOBANCO III PROJECT . Acta Hortic. 784, 127-132
DOI: 10.17660/ActaHortic.2008.784.18
https://doi.org/10.17660/ActaHortic.2008.784.18
genotype, genetic, Castanea sativa
English

Acta Horticulturae