THE USE OF SSR MARKERS TO SCREEN NEW ACCESSIONS BEFORE THEIR INCORPORATION INTO FIG GERMPLASM COLLECTIONS
The fig collection in the SIDT of Badajoz (Spain) contains 220 accessions from different countries, 94% of them local accessions from diverse regions in Spain. As a first step to optimize the management of the collection, homonymies and synonymies have been studied in order to reduce the number of accessions represented. In a second step, since this is a dynamic collection, putative new accessions are studied with SSR markers prior to their introduction in the collection and the amplification patterns compared with the current accessions already conserved. In this work, we describe the process followed to include new accessions from fig germplasm prospections in the Canary Islands and in Aragon. A total of 43 accessions from the Canary Islands, 10 from Tenerife and 33 from La Palma, and 48 from Aragon were studied with SSRs. The results of the Canarian accessions indicate that only 13 accessions (7 from La Palma, 5 from Tenerife and a group of accessions from La Palma and Tenerife with the same SSR profile) were different from the genotypes already conserved in the collection. Regarding the accessions from Aragon, only 10 were unique and different from the genotypes already conserved in the collection and two pairs of accessions were indistinguishable but different from the accessions already conserved. Consequently, 13 new accessions from the Canary Islands and 12 from Aragon will be included in the collection. These results confirm the low polymorphism present in cultivated fig and the suitability of SSR markers to optimize the management of fig germplasm collections.
Giraldo, E., López-Corrales, M. and Hormaza, J.I. (2008). THE USE OF SSR MARKERS TO SCREEN NEW ACCESSIONS BEFORE THEIR INCORPORATION INTO FIG GERMPLASM COLLECTIONS . Acta Hortic. 798, 165-168
Ficus carica, microsatellites, germplasm management, characterization