STANDARDIZATION OF EXPERIMENTAL PROTOCOLS AND SSR MARKERS FOR THE MANAGEMENT OF FIG GERMPLASM COLLECTIONS
In recent years, several SSR markers have been developed in fig. In order to optimize the conservation and use of fig genetic resources in different countries it will be interesting to agree on the experimental protocols and the SSR loci used. As a first step, in this work we describe the identification with 34 SSRs of a subsample of 16 fig cultivars from two different fig germplasm collections. One is the collection of SIDT in Badajoz (Spain) that currently contains 220 accessions, mainly local Spanish cultivars. The other is the collection of the Conservatoire Botanique National Mediterraneen de Montpellier (France) with 277 accessions. Twenty five of the 34 SSRs produced the same results in the two laboratories using different protocols for DNA extraction, amplification and fragment detection. The results obtained indicate that a subset of carefully chosen SSRs can be useful to standardize the molecular identification of fig cultivars in different germplasm collections.
Giraldo, E., López-Corrales, M., Roger, J.P., Khadari, B., Hochu, I., Santoni, S. and Hormaza , J.I. (2008). STANDARDIZATION OF EXPERIMENTAL PROTOCOLS AND SSR MARKERS FOR THE MANAGEMENT OF FIG GERMPLASM COLLECTIONS. Acta Hortic. 798, 213-216
molecular characterization, germplasm, fig, Ficus carica, SSR