DEVELOPMENT OF A GENETIC LINKAGE MAP FOR GENES ASSOCIATED WITH RESISTANCE AND SUSCEPTIBILITY TO FUSARIUM OXYSPORUM F. SP. CUBENSE FROM AN F1 HYBRID POPULATION OF MUSA ACUMINATA SSP. MALACCENSIS
Conventional banana breeding for resistance to Fusarium wilt is hampered by sterility, triploidy, long generation time and the lack of appropriate testing sites. Biotechnological advances involving embryo rescue culture, rapid micropropagation, somatic embryogenesis and molecular marker-assisted selection (MAS) breeding provide opportunities to develop cultivars adapted to changing environments. Wild, seeded Musa acuminata ssp. malaccensis has been found to be resistant to Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 and hence could be useful in the study of populations for the development of molecular markers and gene cloning. 607 AFLP markers have been generated from 14 EcoRI/Mse1 primer combinations and 39 PstI/MseI primer combinations in an F1 hybrid population of a controlled cross between a selected resistant male and a susceptible female. 166 markers were found to be present in the resistant parent, but absent in the susceptible female. 215 markers have been found to be present in the susceptible female, but absent in the resistant parent. Another 226 markers were present in both parents. All these markers segregated in F1 hybrids are potentially very useful for MAS. Two linkage maps for putative resistance and susceptibly markers to Foc have been developed from data obtained.
Kayat, F., Bonar, N., Waugh, R., Rajinder, S., Rahimah, A.R., Rashid, K.A. and Othman, R.Y. (2009). DEVELOPMENT OF A GENETIC LINKAGE MAP FOR GENES ASSOCIATED WITH RESISTANCE AND SUSCEPTIBILITY TO FUSARIUM OXYSPORUM F. SP. CUBENSE FROM AN F1 HYBRID POPULATION OF MUSA ACUMINATA SSP. MALACCENSIS. Acta Hortic. 828, 333-340
banana, resistance breeding, Fusarium wilt, 'tropical race 4', screening