COMMERCIAL MASS CLONAL PROPAGATION OF GRAPEVINE CV. 'BIDANEH SEFID': IN VITRO FRAGMENTED SHOOT APEX CULTURE OF AN ELITE GERMPLASM
With the aim of developing a rapid and reliable commercial technique for mass clonal propagation of pathogen-free plants of Vitis vinifera cv. Bidaneh Sefid, a unique in vitro propagation method using fragmented shoot apices was examined. Primary leaf-like structures obtained from shoot apices fragmented in liquid culture, were successfully cultured in vitro in MS basal solid medium containing 2.0 mg L-1 BA. Induced shoots were sub-cultured regularly and the plantlets obtained were rooted in hormone-free 1/2 MS medium. Plantlets were acclimatized and transferred to glasshouse successfully. This technique has unique potential to produce commercially large numbers of plants through direct organogenesis in a short period of time. It could also be a cost-effective way to mass produce clones of rare and elite plant germplasm derived from meristem-tip culture or genetic transformation.
Salami, S.A., Ebadi, A. and Zamani, Z. (2009). COMMERCIAL MASS CLONAL PROPAGATION OF GRAPEVINE CV. 'BIDANEH SEFID': IN VITRO FRAGMENTED SHOOT APEX CULTURE OF AN ELITE GERMPLASM. Acta Hortic. 829, 383-388
Vitis, mass propagation, germplasm, transformation