APPLICABILITY OF ISSRS FOR GENOTYPE IDENTIFICATION IN A TOMATO BREEDING COLLECTION
The ISSR (Inter-Simple Sequence Repeats) technique was applied to a set of wild species, modern varieties and F1 tomato hybrids.
Genotype identification is of great importance for seed production and variety propagation where the UPOV system still relies on phenotypic markers.
The inherited instability of these markers often leads to disputes between seed producers, traders and farmers.
The problem was aggravated by the introduction of molecular markers in plant breeding that was witnessed in the recent years.
Marker-assisted selection (MAS) is becoming a tool that brings major advantages for the seed companies and they are readily adopting it in their breeding programs.
Modern genetic technology allows for identification of genotypes based on the unique sequences in their genome.
This opens the possibility for effective development of a system for variety identification.
The aim of the present report is to present the results from testing ISSRs as candidate technique for identification of tomato genotypes with the assistance of highly polymorphic molecular markers.
A specific profile was obtained for every accession from the group of wild species while the differences between the domesticated forms are considerably less.
The prospects of adoption of ISSRs by breeders and producers as well as their potential and shortcomings if proposed for inclusion in the standard DUS testing are discussed.
Bojinov, B.M. and Danailov, ZH.P. (2009). APPLICABILITY OF ISSRS FOR GENOTYPE IDENTIFICATION IN A TOMATO BREEDING COLLECTION. Acta Hortic. 830, 63-70
DOI: 10.17660/ActaHortic.2009.830.6
https://doi.org/10.17660/ActaHortic.2009.830.6
DOI: 10.17660/ActaHortic.2009.830.6
https://doi.org/10.17660/ActaHortic.2009.830.6
Lycopersicon esculentum, molecular markers, varieties, phenotypic uniformity, DUS testing, heterogeneity
English
830_6
63-70