MICROPROPAGATION OF CORYLUS COLURNA L.

I.S. Kosenko, A.L. Boyko, A.I. Opalko, M.V. Nebykov, G.A. Tarasenko
Corylus colurna L. micropropagation results are shown. The basal nutrient medium of Murashige and Skoog, which enabled rapid formation of new spears and roots in shoot apices, excised from buds of representative spears of C. colurna L., were developed. The data obtained prove that at the stage of C. colurna L. explant establishment into the in vitro culture 0,1 mg/L kinetin concentration against a background of half the number of inorganic substances from the MS-base content and 0,01 mg/L IAA favoured an explant culture. On the proliferation phase, substitution of kinetin for BАP and IAA for IBA, addition of 1,0 mg/L gibberellic acid A3, introduction of double Fe-helat into the nutrient medium MS with 0,1 mg/L BАP (instead of kinetin) and 0,01 mg/L IAA ensured the best morphogenesis and shoots growth. Root formation proved to be the most efficient on kinetin-free nutrient medium MS, which contained, mg/L: 0,1 - BAP, 0,8 - IBA and 1,0 - gibberellic acid A3. The combination of 0,1 mg/L 2,4-D with 0,01 mg/L BAP was effective for root formation, but less effective than complex MS with BAP, IBA and gibberellic acid A3. Thiamine•HCl in 0,5 and 1,0 mg/L concentrations expedited C. colurna L. explant establishment into the in vitro culture, proliferation and morphogenesis. There is an opinion that adenine sulfate•dihydrate is the essential element of nutrient medium for the in vitro culture, but a positive effect of this complex was received only in a small concentrations 0,5 and 1,0 mg/L which was much lowers than base level MS. Among the elements of C. colurna L. micropropagation technology, the most important is the growing of test-tube implants and their adaptation to unsterile ex vitro conditions.
Kosenko, I.S., Boyko, A.L., Opalko, A.I., Nebykov, M.V. and Tarasenko, G.A. (2009). MICROPROPAGATION OF CORYLUS COLURNA L.. Acta Hortic. 845, 261-266
DOI: 10.17660/ActaHortic.2009.845.37
https://doi.org/10.17660/ActaHortic.2009.845.37
Corylus сolurna L., explant, filbert, in vitro culture, micropropagation, nutrient medium, regenerated plants
English

Acta Horticulturae