GENETIC AND EPIGENETIC STABILITY OF HUMULUS LUPULUS AFTER IN VITRO PROCEDURES

E.L. Peredo, R. Folgado, M.A. Revilla, R. Arroyo-García
Somaclonal variation can be a major problem during in vitro procedures. Recovering true-to-type plants are the main goal of cryopreservation, cold storage, micropropagation, and, usually, in adventitious regeneration. In the present work the (epi)genetic stability of these techniques is evaluated using several molecular markers as RAPD, REMAP, AFLP, and MSAP. A total of 315 plants, from 53 cultivars and wild accessions were analysed. No genetic variation was detected in any of the analysed plants. Epigenetic changes, most of them corresponding to DNA demethylations, were detected in plants recovered from each technique. The majority of the variation observed corresponded to the in vitro introduction step needed for each of the techniques. Some of the variation in the treated samples was attributed to each in vitro technique while some was caused by singletons. Also, when the time of culture was extended an increase of variation in the micropropagation and adventitious regeneration techniques was detected, measured as higher genetic distance to the control samples.
Peredo, E.L., Folgado, R., Revilla, M.A. and Arroyo-García, R. (2009). GENETIC AND EPIGENETIC STABILITY OF HUMULUS LUPULUS AFTER IN VITRO PROCEDURES. Acta Hortic. 848, 115-124
DOI: 10.17660/ActaHortic.2009.848.12
https://doi.org/10.17660/ActaHortic.2009.848.12
amplified fragment length polymorphism (AFLP), hop, methylation sensitive amplified polymorphism (MSAP), randomly amplified polymorphic DNA (RAPD), retrotransposon microsatellite amplified polymorphism (REMAP), somaclonal variation
English

Acta Horticulturae