LONG-TERM SUBCULTURE AND MAINTENANCE OF TRANSGENIC RESISTANT EMBRYOGENIC CALLUS IN LITCHI (LITCHI CHINENSIS SONN.)

The effects of subculture method, hygromycin, AgNO₃, inoculation quantity and subculture cycle on the growth of litchi transgenic resistant embryogenic callus (TREC) were studied in this experiment. Meanwhile, the influences of the medium and long-term subculture on the GUS expression of litchi resistant embryogenic callus were carried out. The obtained results showed that litchi TREC could be steadily maintained for a long time when they were alternately subcultured in the MS medium containing 1.0 mg/L 2,4-D, 50 mg/L hygromycin and the MS medium supplemented with 1.0 mg/L 2,4-D, 100 mg/L inositol and 50 mg/L hygromycin.