POD ISOENZYME AND RAPD ANALYSIS ON LITCHI IN FUQING

L.S. Liu, D.M. Pan, F.L. Zhong, J.B. Wang
The paper discussed the genetic relationship between superior individual late-maturity Yurongwanli and other cultivars. To do so POD isoenzyme and RAPD technology were used to analyze litchi germplasm resources of Fuqing city. There were 4 bands produced by POD isoenzyme electrophoresis. According to the differences of these bands, Shishengshu1, Shishengshu2, Xiafanzhi and Dongliuyihao were put in the frist category, while Shangfanzhi and Yuanhong were placed in the second category. RAPD-PCR analysis showed that seven primers produce a total of 59 bands, with 8.43 fragments per prime. Among these 59 bands 40 were polymorphic, with a 67% polymorphism rate. The DPS2000 software was used to analyze the RAPD genotype date matrix. The result showed that the 7 cultivars can be divided into two categories when the genetic distance D value is 0.70 and four categories when the D value is 0.60. These two results indicated that Yurongwanli has the closest genetic relationship with Hongxiuqiu.
Liu, L.S., Pan, D.M., Zhong, F.L. and Wang, J.B. (2010). POD ISOENZYME AND RAPD ANALYSIS ON LITCHI IN FUQING. Acta Hortic. 863, 189-194
DOI: 10.17660/ActaHortic.2010.863.24
https://doi.org/10.17660/ActaHortic.2010.863.24
litchi, POD isoenzyme, RAPD analysis, cluster analysis
English

Acta Horticulturae