ASSESSMENT OF DIFFERENT MEDIA FOR THE PRODUCTION OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA TO CONTROL THE RED PALM WEEVIL RHYNCHOPHORUS FERRUGINEUS (OLIV.)

G. Hegazy, S.T. Shehata, M.A. Salem, F.M. Mariy, A.A. Aldossary
The growth of the entomopathogenic fungus Beauveria bassiana (B-SA3) in different media and subsequent quantity and viability of produced conidia was determined as well as virulence against the red palm weevil R. ferrungineus. Five ingredients in agar were tested; wheat, corn, barley, oat and soy bean. The standard SDYA medium was used for comparison.
The yield of cultured conidial was highest on media prepared by wheat followed by oat and least on barley. Viability of harvested conidia from all of the tested media, as determined by percentage germination ranged between 94.03-97.6% and relatively comparable with those obtained using SDYA. B. bassiana (B-SA3) conidia produced on the tested media although highly infective to R. ferrungineus adults was significantly less than that harvested from SDYA as exhibited by the calculated LC50 and LT50. Of the considered ingredients, conidia cultured on wheat medium surpassed those produced on the other media (LC50 was 2.11×107 conidia/ml) followed by soy bean (1.06×108 conidia/ml). Least infectivity to the red palm weevil was by conidia produced on oat or corn medium, as LC50 was 2.81×108 and 3.37×108 conidia/ml, respectively. Mycosis was apparent on cadavers by the 7th day following death of all treated weevils.
Hegazy, G., Shehata, S.T., Salem, M.A., Mariy, F.M. and Aldossary, A.A. (2010). ASSESSMENT OF DIFFERENT MEDIA FOR THE PRODUCTION OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA TO CONTROL THE RED PALM WEEVIL RHYNCHOPHORUS FERRUGINEUS (OLIV.). Acta Hortic. 882, 1015-1026
DOI: 10.17660/ActaHortic.2010.882.118
https://doi.org/10.17660/ActaHortic.2010.882.118
Beauveria bassiana, culture, media, conidia, Rhynochophorus ferrungineus, red palm weevil, bioassay
English

Acta Horticulturae