EVALUATION OF SHARKA RESISTANCE IN NEW CULTIVARS AND SELECTIONS OF THE APRICOT BREEDING PROGRAMME AT CEBAS-CSIC IN MURCIA (SPAIN)
The Region of Murcia is one of the main apricot growing areas in Spain. In these areas, apricot culture is being seriously affected by sharka disease, caused by Plum pox virus. So far, the resistance evaluation process has been mainly carried out in sealed greenhouses. However, due to difficulties in evaluation methodology in control conditions, it is necessary to complement this resistance test with field assays to ensure an accurate evaluation as well as to have the opportunity to evaluate the incidence of sharka on fruits. In this study, 13 new cultivars and 32 selections of CEBAS-CSIC have been grafted onto diseased trees. Observation of visual symptoms in leaves and fruits was done, and later confirmed by ELISA and RT-PCR. We have compared the field results with the greenhouse and quarantine shelter assays carried out in parallel. We suggest classifying the response to PPV in four groups, advising farmers about the level of resistance of new cultivars as follows: very susceptible, susceptible, partially resistant and very resistant. The resistance of cultivars Rojo Pasión, Murciana, Mirlo Blanco, Mirlo Naranja and Mirlo Rojo to PPV has been confirmed to date, and thus offers an alternative for the threatened apricot culture. The controversial results obtained in the different tests performed in field, greenhouse and quarantine shelter, as well as the expression of symptoms on leaves and fruits, will be also discussed.
Rubio, M., Dicenta, F., Martínez-Gómez, P., Egea, J. and Ruiz, D. (2011). EVALUATION OF SHARKA RESISTANCE IN NEW CULTIVARS AND SELECTIONS OF THE APRICOT BREEDING PROGRAMME AT CEBAS-CSIC IN MURCIA (SPAIN). Acta Hortic. 899, 95-101
Prunus, Plum pox virus, resistance, evaluation, greenhouse, breeding