INHERITANCE OF SILENCING IN TRANSGENIC PLUMS

M. Ravelonandro, R. Scorza, P. Briard, B. Lafargue, R. Renaud
We have studied the heritability of the virus transgene engineered in ‘HoneySweet’ plum through different cross-hybridization with two commercial cultivars of Prunus domestica (‘Prunier d’Ente 303’ and ‘Quetsche 2906’) and one wild species, P. spinosa 2862 rootstock using ‘HoneySweet’ plum as the pollen donor. Over all crosses 46% of the progeny were transgenic. Chi square analyses indicated that the transgene was inherited as a single dominant allele. Transgenic hybrids were challenged with Plum pox virus (PPV) and resistance was analyzed through more than three dormancy cycles. Transgenic hybrids were highly resistant to sharka disease while the untransformed sibling hybrids were susceptible to PPV. As in the ‘HoneySweet’ parent, silencing was shown to be the mechanism of resistance. These results confirmed the high potential of ‘HoneySweet’ plum for PPV resistance breeding programmes.
Ravelonandro, M., Scorza, R., Briard, P., Lafargue, B. and Renaud, R. (2011). INHERITANCE OF SILENCING IN TRANSGENIC PLUMS. Acta Hortic. 899, 139-144
DOI: 10.17660/ActaHortic.2011.899.18
https://doi.org/10.17660/ActaHortic.2011.899.18
silencing, transgenic, Prunus domestica, P. spinosa, Plum pox virus
English

Acta Horticulturae