STUDY OF SLOW GROWTH CONDITIONS OF RANUNCULUS IN VITRO SHOOTS
Micropropagation of Ranunculus is usually carried out to produce plants which form tuberous roots at the end of the in vivo culture cycle. Due to the fact that Ranunculus is very sensitive to photoperiod, it is mandatory that, under Mediterranean conditions, the acclimatization phase can be carried out in a very short period (2-3 months). The large scale commercial propagation is then forced to produce a large amount of plant material in a very short timeframe. In this study, we evaluated slow growth of in vitro Ranunculus plantlets by means of low temperature (2°C) and reduction of oxygen supply (2% O2 and 4% CO2). Low temperatures were able to slow down and control the growth rate of cultures; Ranunculus shoots could survive the storage conditions both at rooting and multiplication stages. Low temperatures stimulated the rooting process for both types of shoots. Evolution of shoot growth after the storage period infers that Ranunculus explants tolerate in vitro cold preservation for three months of storage, although two months seem to be more appropriate for providing a quality shoot production. An hypoxic regime had no effect on the growth of shoots during cold storage or on in vivo survival or agronomical performance of the stored plantlets.
Beruto, M., Rinino, S., Bisignano, A. and Fibiani, M. (2011). STUDY OF SLOW GROWTH CONDITIONS OF RANUNCULUS IN VITRO SHOOTS . Acta Hortic. 908, 391-403
in vitro cold storage, in vitro hypoxic storage, micropropagation, Ranunculus asiaticus