IN VITRO INDUCTION OF OCTOPLOID PLANTS FROM TETRAPLOID ACTINIDIA ARGUTA

C. Liu, X. Sun, H. Dai, Z. Zhang
Actinidia arguta produces greenish-yellow delicious fruits rich in vitamin C. To increase the fruit size of A. Arguta, octoploid plants were induced successfully treating in vitro leave pieces of tetraploid plants with colchicine. Leaf pieces excised from in vitro shoots were treated with 15 different concentrations of colchicine in liquid MS medium supplemented with 6.66 μM BA and 0.64 μM IBA for 3, 5 or 7 days, respectively. The results showed that the bud regeneration rate was affected by the concentration of colchicine and duration of treatment. High concentrations and longer durations reduced the number of regenerated buds. The optimum condition for inducing octoploids was to treat leaf pieces with 0.009% colchicine for 5 days. Cytological analysis established that the chromosome number of tetraploid plants of A. arguta was 2n=4x=116 and that of octoploid plants was 2n=8x=232. Among the 111 regenerated plants from leave pieces treated with colchicine, 30 plants were confirmed as octoploid by chromosome counts and flow cytometry.
Liu, C., Sun, X., Dai, H. and Zhang, Z. (2011). IN VITRO INDUCTION OF OCTOPLOID PLANTS FROM TETRAPLOID ACTINIDIA ARGUTA. Acta Hortic. 913, 185-190
DOI: 10.17660/ActaHortic.2011.913.23
https://doi.org/10.17660/ActaHortic.2011.913.23
chromosome doubling, colchicine, ploidy, tissue culture
English

Acta Horticulturae