PRELIMINARY LOCALIZATION OF FLUORESCENT EST-SSR MARKER RELATED TO DWARFING CHARACTERISTICS IN ACTINIDIA CHINENSIS PLANCH.

X. Xu, C.Y. Jiang, Q.Q. Gu, C.H. Huang, Y.L. Yang
The segregating progenies from the cross between the dwarf cultivar Actinidia chinensis Planch ‘Ganmi 5’ and the normal cultivar ‘Fengxiong 2’ were employed to identify the molecular markers linked to the dwarfing gene of Actinidia by using highly effective simple sequence repeats (SSRs) markers based on expressed sequence tags (ESTs) combining with the method of bulked segregation analysis (BSA) on the automated fluorescent-labeled system (ABI3130). The results showed that a polymorphic co-dominanat marker EST-Ad042 was found to be tightly linked to the dwarfing gene in A. Chinensis. The marker was dominant in the dwarf phenotype and recessive in the non-dwarf phenotype. Further genetic linkage analysis showed the genetic distance between the EST-SSR marker and the dwarfing gene was 8.8 cM. The specific marker of Actinidia not only could be effective in marker-assisted selection for the dwarfing character, but also provide a solid basis for the dwarfing gene mapping and cloning in Actinidia breeding programs.
Xu, X., Jiang, C.Y., Gu, Q.Q., Huang, C.H. and Yang, Y.L. (2011). PRELIMINARY LOCALIZATION OF FLUORESCENT EST-SSR MARKER RELATED TO DWARFING CHARACTERISTICS IN ACTINIDIA CHINENSIS PLANCH.. Acta Hortic. 913, 235-240
DOI: 10.17660/ActaHortic.2011.913.30
https://doi.org/10.17660/ActaHortic.2011.913.30
'Ganmi 5', kiwifruit, molecular markers
English

Acta Horticulturae