CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV. ACTINIDIAE POPULATIONS USING DIFFERENT TYPING TECHNIQUES

A. Mazzaglia, M. Renzi, M.C. Taratufolo, G.M. Balestra
Over the last few years a severe bacterial disease on kiwifruit has been reported in Italy; its causal agent was identified as Pseudomonas syringae pv. actinidiae. For epidemiological purposes, we evaluated the discriminatory power of various PCR-based fingerprinting approaches; profiles were generated by rep-PCR (including also (GTG)5-PCR), IS50-PCR, and RAPD, from a wide collection of Italian, Korean and Japanese strains, to investigate the genetic relationships between them and also with some representative strains of related pathovars. It wasn’t possible to find dissimilarities among Italian strains, whilst some were evidenced between these and the Japanese/Korean strains of this pathovar. Also, the other pathovars clustered separately, with the pathovar theae being always more closely related to actinidiae than the others. In general, the results obtained with the different methods were in good agreement, with RAPD showing the highest values of divergence between groups.
Mazzaglia, A., Renzi, M., Taratufolo, M.C. and Balestra, G.M. (2011). CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV. ACTINIDIAE POPULATIONS USING DIFFERENT TYPING TECHNIQUES. Acta Hortic. 913, 495-499
DOI: 10.17660/ActaHortic.2011.913.66
https://doi.org/10.17660/ActaHortic.2011.913.66
kiwifruit, bacterial canker, genetic fingerprinting, RAPD, rep-PCR
English

Acta Horticulturae