RASPBERRY CRYOPRESERVATION BY DROPLET VITRIFICATION TECHNIQUE
We applied a cryopreservation method utilizing droplet vitrification to in vitro grown shoot apices of raspberry Latham (Rubus idaeus L.). Excised meristems were transferred into the loading solution for 20 min at room temperature, then were treated with the vitrification solution (PVS2) for 30 min at 0°C and finally frozen into individual droplets of PVS2 placed on a strip of aluminium foil. After 30 min in LN, cryopreserved explants were rapidly re-warmed in the recovery solution for 15 min and subsequently placed onto a regeneration medium where they were kept in darkness. After 7 days the explants were transferred to a fresh medium and cultured under conditions similar to those of regular in vitro grown shoots. Survival/regrowth was observed 9 days after being transferred onto the regeneration medium and shoots with 2-4 leaves were observed after 18 days on 18% of the explants. Although further experiments are necessary for the optimisation of the protocol, these preliminary results show the potential applicability of this technique for the conservation of raspberry genetic resources.
Condello, E., Ruzić, D., Panis, B. and Caboni, E. (2011). RASPBERRY CRYOPRESERVATION BY DROPLET VITRIFICATION TECHNIQUE. Acta Hortic. 918, 965-969
in vitro preservation, regrowth, Rubus idaeus