CRYOPRESERVATION OF PEACH SHOOT TIPS BY ENCAPSULATION DEHYDRATION
Shoot tips of peach cultivars Summer Grand, San Giorgio and Babygold 6 were cryopreserved by encapsulation dehydration. Tips were pre-cultured for 1, 3, 5 or 7 days in liquid medium with increasing sucrose concentrations (0.5, 0.75, 1.0 or 1.25 M). Desiccation was performed with silica gel for 0, 4, 6, 9, 14, 20 or 24 hours. The highest regrowth, from 33 to 46% depending on the cultivar, was observed with a pre-culture of encapsulated tips for 3 days on 0.5 M sucrose enriched liquid medium and a desiccation of beads in silica gel for 9 hours prior to immersion in liquid nitrogen.
Damiano, C., Sgueglia, A., Arias, M., Frattarelli, A., Condello , E. and Caboni, E. (2011). CRYOPRESERVATION OF PEACH SHOOT TIPS BY ENCAPSULATION DEHYDRATION . Acta Hortic. 918, 121-124
liquid nitrogen, Prunus persica (L.) Batsch, shoot tips, silica gel, sucrose