PRODUCTION AND IDENTIFICATION OF F1 HYBRIDS BETWEEN RAPE-RADISH ADDITION LINE D AND BRASSICA OLERACEA

Yunhua Ding, Holger Budahn, Qian Li, Lijun Li, Yuancai Jian, Jungen Kang, Li Li
To transfer radish chromosome d carrying resistance against nematode Heterodera schachtii (Schmidt) to Brassica oleracea, sexual hybridization between disomic rape-radish addition line d (2n=38+2) as chromosome d donor and cabbage as recipient (2n=18) was performed. F1 hybrids were obtained by means of embryo rescue. They were identified by morphological investigation, cytological and molecular marker techniques. The results indicated that the tested F1 plants had the same morphological characters, partly resembling female parent, partly like male parent. The presence of chromosome d in F1 plants was tested by the chromosome d specific RAPD marker OPF07-363 which was developed in a former project. All F1 plants presented the chromosome d specific band. Furthermore, three SSR markers were used to identify the hybrid character of F1 plants. As a result, all investigated plants were confirmed as true hybrids. Cytological observation of somatic cells showed that the F1 contained 29 chromosomes. Therefore, transfer of radish chromosome d from disomic rape-radish addition line d to cabbage was successful and the F1 hybrids can be used as basic material for further backcrosses with cabbage.
Yunhua Ding, , Holger Budahn, , Qian Li, , Lijun Li, , Yuancai Jian, , Jungen Kang, and Li Li, (2011). PRODUCTION AND IDENTIFICATION OF F1 HYBRIDS BETWEEN RAPE-RADISH ADDITION LINE D AND BRASSICA OLERACEA. Acta Hortic. 918, 611-617
DOI: 10.17660/ActaHortic.2011.918.76
https://doi.org/10.17660/ActaHortic.2011.918.76
rape-radish addition line, cabbage, interspecific hybrid
English

Acta Horticulturae