GENETIC DIVERSITY OF RED SKINNED CHINESE SAND PEAR CULTIVARS REVEALED BY AFLP MARKERS
A total of 38 Chinese sand pear cultivars and landraces (mostly red skinned types) native to southwestern China were subjected to Amplified Fragment Length Polymorphism (AFLP) analysis. The six primer combinations (EcoRI-AAG/MseI-CAT, EcoRI-ACT/MseI-CTT, EcoRI-AAC/MseI-CAG, EcoRI-AAC/MseI-CTA, EcoRI-AAC/MseI-CAA, EcoRI-AAG/MseI-CTC) generated 443 fragments from the 38 pear accessions, of which 356 fragments were polymorphic. The percentage of polymorphism for the six primer combinations was from 76 to 85% with a mean of 80%. Primer EcoRI-ACT/MseI-CTT showed the highest number of polymorphic fragments, percentage of polymorphism and marker index. The six primer combina-tions successfully distinguished 32 cultivars, of which one pair of cultivars might be homonyms. Three pairs of cultivars could not be differentiated; these might be synonyms or bud mutants. Dendrogram analysis clustered the accessions into five groups. Huobali types, a very common name referring to red pears harvested in late July and early August in Yunnan Province, were scattered in different groups, revealing the possibility of different origins. The red-skinned sand pears clustered with the non-red skinned sand pears, which inferred that the red-skinned sand pears might have originated from bud mutations or open-pollinated seedlings of non-red skinned sand pears.
Dong Zhang , , Yuanwen Teng, and Lu Bo, (2011). GENETIC DIVERSITY OF RED SKINNED CHINESE SAND PEAR CULTIVARS REVEALED BY AFLP MARKERS. Acta Hortic. 918, 679-684
red Chinese sand pear, AFLP, genetic diversity