MOLECULAR CHARACTERIZATION OF PRUNUS ACCESSIONS OF TRADITIONAL CULTIVARS PROSPECTED IN WESTERN ANDALUSIA, SPAIN

P. Rallo, C. López, M.P. Suárez, A.M. Morales-Sillero, L. Casanova , R. Jiménez, A. Sánchez , J.R. Guzmán
Forty-six accessions of Prunus spp. belonging to 19 presumably local cultivars have been prospected in different orchards located in Olivares, Constantina (Sevilla) and Galaroza (Huelva) in Spain, within the frame of the project INIA RF2007-00027-C06-05. DNA was isolated from leaf samples and analysed with a set of 8 SSR loci previously selected by Wünsch (2009) for its good performance and transferability in different Prunus species. All 19 cultivars were fully discriminated. No cases of synonym or homonym were found, i.e., all samples from different trees of the same cultivar were correctly assigned. The dendrogram obtained with the UPGMA clustering method and Dice coefficient shows five different groups according to the different species: peach, apricot, plum (2 clusters) and cherry. The plum group was the more numerous as well as the more complex one: multiple amplification products (4-6 alleles/locus and sample) reveal the polyploid character of most of the samples.
Rallo, P., López, C., Suárez, M.P., Morales-Sillero, A.M., Casanova , L., Jiménez, R., Sánchez , A. and Guzmán, J.R. (2011). MOLECULAR CHARACTERIZATION OF PRUNUS ACCESSIONS OF TRADITIONAL CULTIVARS PROSPECTED IN WESTERN ANDALUSIA, SPAIN. Acta Hortic. 918, 685-688
DOI: 10.17660/ActaHortic.2011.918.87
https://doi.org/10.17660/ActaHortic.2011.918.87
genetic resources, local cultivars, SSR, dendrogram, Prunus domestica L., plum, peach, apricot, cherry
English

Acta Horticulturae