SIMPLE SPECTROGRAPHIC TECHNIQUE USING A FLUORESCENT DYE TO EVALUATE WATER-UPTAKE BY CUT FLOWER PEDUNCLES
A low-cost, simple spectrographic technique was developed using a commercial camera and fluorescent dye (excitation peak of wavelength: 494 nm, emission peak of wavelength: 521 nm) to monitor water uptake by a cut flower stem. To obtain a clear image of fluorescence emission, the following combinations of light sources, exciter filters, and emitter filters were compared: (1) halogen light with a fiber illuminator for microscopes was tested against speedlight for a commercial camera, (2) a blue dichroic filter versus an exciter filter for fluorescent reagent GFP, and (3) band-pass filters with a center wavelength of 515 nm versus those with a center wavelength of 520 nm. Cut rose flowers were used as samples. The clearest image was obtained when a combina¬tion of speedlight, exciter filter for a fluorescence microscope, and emitter filter with a center wavelength at 520 nm was used.
Matsushima, U., Yamada, Y., Shono , H. and Okada, M. (2011). SIMPLE SPECTROGRAPHIC TECHNIQUE USING A FLUORESCENT DYE TO EVALUATE WATER-UPTAKE BY CUT FLOWER PEDUNCLES. Acta Hortic. 919, 35-38
storage, optical filter, freshness