AN EFFICIENT IN VITRO ESTABLISHMENT AND PLANT REGENERATION FOR CARICA PAPAYA 'MARADOL'

R. Salgado-Garciglia, L. Carreto-Montoya, M.P. Chávez-Moctezuma, A. Hernández-García, L.M. Suárez-Rodríguez , R. López-Gómez
The objective of this work was to develop an efficient in vitro establishment and plant regeneration for Carica papaya ‘Maradol’. Shoot tips and lateral buds collected from 45-days-old non-aseptic seedlings were cultured on Murashige and Skoog (MS) medium. The roles of antibiotics (ampicillin, chloranphenicol and trimetoprim-sulphamethoxazole), and silver tiosulphate (STS) were investigated to obtain bacterial free explants and high tissue viability. Among the antibiotics supplemented to MS, the trimetoprim-sulphamethoxazole was the most effective to inhibit the endophytic bacteria contamination. Several growth regulators were also investigated for callus induction, shoot proliferation and somatic embryos forma¬tion. Callus was induced on MS added with 0.5 mg/L naphthalene acetic acid (NAA), and 0.5 mg/L benzyladenine (BA). The highest efficiency of shoot proliferation was observed in 0.1 mg/L NAA and 0.1 mg/L BA. These media were added with 1.0 µM STS. The somatic embryos were formed in MS with 0.4 mg/L glutamine, 10 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The best condition for rooting was half-strength MS medium supplemented with 0.1 mg/L of indole-3-butyric acid (IBA). The best survival of plantlets (90%) was obtained in the potting mix, peat moss:agrolite (1:1, v/v). All plantlets grew and exhibited normal development.
Salgado-Garciglia, R., Carreto-Montoya, L., Chávez-Moctezuma, M.P., Hernández-García, A., Suárez-Rodríguez , L.M. and López-Gómez, R. (2011). AN EFFICIENT IN VITRO ESTABLISHMENT AND PLANT REGENERATION FOR CARICA PAPAYA 'MARADOL'. Acta Hortic. 923, 189-194
DOI: 10.17660/ActaHortic.2011.923.27
https://doi.org/10.17660/ActaHortic.2011.923.27
micropropagation, papaya, shoot proliferation, somatic embryogenesis
English

Acta Horticulturae