ISOLATION AND ANALYSIS OF 5' REGULATORY SEQUENCE OF VVICOR11 FROM COWBERRY
The gene of vvicor11 isolated from cowberry is a KS type dehydrin gene, which belongs to LEA D11 or LEA II protein family. LEA (late embryogenesis-abundant protein) proteins accumulate during dehydration, low temperature, freezing, abscisic acid (ABA) treatment, and seed maturation. To better understand its expression and regulation, we isolated an 1110 bp 5 region upstream starting site of the putative vvicor11 (GenBank No. FJ429387). The sequence was identified using a genome walking strategy based on the cDNA sequence of cowberry vvicor11. Computational analysis using the PLACE and PlantCARE databases showed that the sequence included 5-UTR Pyrimidine-rich motifs and typical promoter characterization structures, such as a TATA-box and a CAAT-box, to ensure the proper transcription initiation. There were many potential cis-acting elements involved in stress, such as ABRE, LTR, TC-rich repeats, ARE, MYB (MYC), TGACG-motif, CBFHV, and so on. The presence of these motifs could suggest that the expression of vvicor11 could be induced by ABA, low temperature, drought and high salt stress, and could be a downstream target gene of the CBF transcription factor. Transient expression of a 5- regulatory sequence deletion series showed that the degree of 5- regulatory sequence activation was increased the longer the sequences. One of the constructions, the CP5::GUS construct was expressed in the root, stem, leaf, petal and style of tobacco without inductive treatment. This suggested that the 5- regulatory sequence possessed at least basal expression in various organs of a different species.
Wang, Q.J., Gao, Z.H., Tao, J.M., Qu, S.C. and Zhang, Z. (2012). ISOLATION AND ANALYSIS OF 5' REGULATORY SEQUENCE OF VVICOR11 FROM COWBERRY. Acta Hortic. 926, 107-111
Vaccinium vitis-idaea, 5' regulatory sequence of vvicor11, Agrobacterium- mediated transformations, tobacco, transient expression