EMBRYOGENIC CAPACITY OF EMBRYO-DERIVED EXPLANTS FROM DIFFERENT OLIVE CULTIVARS
The olive tree (Olea europaea L.) is difficult to manipulate in vitro; however, when somatic embryogenesis is observed, it has generally occurred in zygotic embryo derived explants. In this research, the embryogenic capacity of zygotic explants of Picual, Arbequina (Spanish cultivars), Dahbia and Moroccan Picholine (Moroccan cultivars), was investigated. Radicle and cotyledons were cultivated in OMc medium supplemented with 25 µM IBA and 2.5 µM 2iP for three weeks, transferred to OMc medium for four weeks and finally, to ECO medium supplemented with 0.25 µM IBA, 0.5 µM 2iP and 0.44 µM BAP. As expected, genotype had a strong influence on the embryogenic capacity with Arbequina showing the highest response, both on radicles (13%) and cotyledons (17%), followed by Dahbia (9% on radicles and 7% on cotyledons). Picual gave positive response only from radicle explants (7%). Moroccan Picholine did not show any embryogenic response under our experimental conditions.
Mazri, M.A., Belkoura, I., Pliego-Alfaro, F. and Belkoura, M. (2012). EMBRYOGENIC CAPACITY OF EMBRYO-DERIVED EXPLANTS FROM DIFFERENT OLIVE CULTIVARS. Acta Hortic. 929, 397-403
Olea europaea L., in vitro, somatic embryogenesis, 'Picual', 'Arbequina', 'Dahbia', 'Moroccan picholine'